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Differential microRNA Expression between Asthmatic and Healthy Donors
Citation:
JARDIM, M. J., L. A. DAILEY, H. Ren, AND D. DIAZ SANCHEZ. Differential microRNA Expression between Asthmatic and Healthy Donors. Presented at American Thoracic Society Meeting, New Orleans, LA, May 14 - 19, 2010.
Impact/Purpose:
We show here that BECs collected from mild/moderate asthmatic donors have a different baseline miRNA profile when compared to BECs from healthy donors.
Description:
Introduction: Asthma is a chronic lung disease and is pathologically characterized by increases in bronchial hyperresponsiveness and chronic inflammation. It is estimated to affect approximately 300 million people worldwide and cases are expected to continually rise over the next several years. MicroRNAs are small noncoding RNAs that have been quickly established as key regulators of gene expression. As such, miRNAs have been found to be potent regulators of several cellular processes including apoptosis, proliferation and differentiation. Aberrant miRNA expression has been implicated in the pathogenesis of several human maladies such as solid tissue and hematological malignancies, heart disease, congenital organ defects and neurodegenerative diseases. The contribution of miRNA regulated gene expression to the development and pathogenesis ofasthma is currently unresolved. In this study, we hypothesized that miRNAs are differentially expressed in BECs from mild/moderate asthmatic donors. Methods: Bronchial epithelial cells (BECs) from healthy and mild/moderate asthmatic donors were obtained and cultured at air liquid interface. Total RNA was isolated and analyzed for miRNA expression using microarray profile analysis and quantitative realtime
PCR. Putative targets for significantly changed miRNAs were identified using miRBase and miRDB databases. Molecular network analyses were generated with Ingenuity Pathway Analysis. Results: We show here that BECs collected from mild/moderate asthmatic donors have a different baseline miRNA profile when compared to BECs from healthy donors. 297 miRNAs were identified above background in both cell groups. Ofthese, 65 miRNAs (22%) were significantly up-or down-regulated (2:1.5 fold; p-values:::0.05) between these two groups. Molecular network analysis ofputative targets for these miRNAs indicated that they may be involved in regulating the expression ofgenes participating in oxidative stress pathways (p-value