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Characterization of Microbial Population Structures in Recreational Waters and Primary Sources of Fecal Pollution with a Next-Generation Sequencing Approach
Citation:
SHANKS, O. C., S. McLellan, S. M. Huse, AND M. L. Sogin. Characterization of Microbial Population Structures in Recreational Waters and Primary Sources of Fecal Pollution with a Next-Generation Sequencing Approach. Chapter 9, K. Sen and N. J. Ashbolt (ed.), Environmental Microbiology: Current Technology and Water Applications, ISBN: 978-1-904455-70-7. Caister Academic Press, Norwich, Uk, , ,, (2010).
Impact/Purpose:
To inform the public.
Description:
The invention of new approaches to DNA sequencing commonly referred to as next generation sequencing technologies is revolutionizing the study of microbial diversity. In this chapter, we discuss the characterization of microbial population structures in recreational waters and primary sources of fecal pollution using a GS-FLX Pyrosequencer. An overview of the principals of the technology, workflow, sequencing strategies, and data analysis challenges are included. In addition, the bacterial population structure of an untreated wastewater sewage sample is reported and discussed to demonstrate a pyronsequencing approach for studying a microbial community. Emphasis is placed on the application of pyrosequencing technology for recreational water quality management including the discovery of alternative indicators of fecal pollution and improving the link between fecal pollution and the transmission of waterborne disease.
