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Toxicogenomic identification of biomarkers of acute respiratory exposure sensitizing agents
Citation:
PUCHEU-HASTON, C. M., L. B. COPELAND, E. BOYKIN, AND M. D. WARD. Toxicogenomic identification of biomarkers of acute respiratory exposure sensitizing agents. Presented at North Carolina Society of Toxicology, Fall 2009 meeting, RTP, NC, October 13, 2009.
Impact/Purpose:
This abstract describes a research effort to identify biomarkers that differentiate the responses to allergens vs. non-allergens following an acute exposure. Biomarker genes obtained from this analysis will facilitate improvements in methods of screening for respiratory sensitizers.
Description:
Allergy induction requires multiple exposures to an agent. Therefore the development of high-throughput or in vitro assays for effective screening of potential sensitizers will require the identification of biomarkers. The goal of this preliminary study was to identify potential biomarkers that differentiate the response to allergen vs non-allergen agents following an acute exposure in natve individuals. Female BALB/c mice received a single intratracheal aspiration exposure to Metarhizium anisopliae crude antigen (MACA) or bovine serum albumin (BSA) in Hank's Balanced Salt Solution (HBSS) or HBSS alone. Mice were sacrificed after 1, 3, 6, 12, 18 and 24h. Bronchoalveolar lavage fluid (BALF) was evaluated to determine total and differential cellularity, total protein concentration and LDH activity. RNA was isolated from lung tissue for microarray . analysis and RT-PCR.