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Molecular-Based Detection Systems for Cryptosporidium Oocysts
Citation:
VILLEGAS, E. Molecular-Based Detection Systems for Cryptosporidium Oocysts. Presented at 2009 USEPA STAR Grants Workshop on Innovative Approaches to Detecting Microorganisms and Cyanotoxins in Water, Philadelphia, PA, May 20 - 21, 2009.
Impact/Purpose:
The overall objective of this task is the development of improved occurrence detection methods for protozoan parasites and Microsporidia. Since this work is a primary focus of the Branch, this task supports several individual projects related to sample preparation and protozoan detection. Together these projects will lead to complete methods able to support the UCMR and the CCL2 and CCL3.
Description:
The presentation describes on-going studies in collaboration with US EPA Region 2, 3, and the CDC on identifying sources of Cryptosporidium oocyst contamination in source waters using conventional and real-time PCR approaches.