EPA Science Inventory

Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice

Citation:

WARE, M. W. AND E. VILLEGAS. Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice . Veterinary Parasitology. Elsevier, Shannon, Ireland, 168(3-4):329-331, (2010).

Description:

This study evaluates Cryptosporidium parvum oocyst production in dexamethasone suppressed CF-1 and C57BL/6 mice. Both models can yield 1 x 109 total oocysts over a 20 day production period; however, only 20 CF-1 mice are required to reliably achieve this goal compared to 40 C57BL/6 mice. Although oocyst yields per mouse are similar for both mouse strains, the survival rate for CF-1 mice is higher, resulting in reduced lost production time per study when compared to the C57BL/6 mice. This study presents a more efficient and cost effective dexamethasone suppressed murine model of propagating high concentrations of C. parvum oocysts.

Purpose/Objective:

The overall objective of this task is the development of improved occurrence detection methods for protozoan parasites and Microsporidia. Since this work is a primary focus of the Branch, this task supports several individual projects related to sample preparation and protozoan detection. Together these projects will lead to complete methods able to support the UCMR and the CCL2 and CCL3.

URLs/Downloads:

Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice   (PDF,NA pp, 14 KB,  about PDF)

Improved Cryptosporidiumk parvum oocyst propagation using dexamethasone suppressed CF1 mice   (PDF,NA pp, 8 KB,  about PDF)

Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice   (PDF,NA pp, 23 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 03/25/2010
Completion Date: 03/25/2010
Record Last Revised: 04/08/2010
Record Created: 06/16/2009
Record Released: 06/16/2009
OMB Category: Other
Record ID: 210167

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

BIOHAZARD ASSESSMENT RESEARCH BRANCH