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Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice
WARE, M. W. AND E. VILLEGAS. Improved Cryptosporidium parvum oocysts propagation using dexamethasone suppressed CF-1 mice . Veterinary Parasitology. Elsevier, Shannon, Ireland, 168(3-4):329-331, (2010).
This study evaluates Cryptosporidium parvum oocyst production in dexamethasone suppressed CF-1 and C57BL/6 mice. Both models can yield 1 x 109 total oocysts over a 20 day production period; however, only 20 CF-1 mice are required to reliably achieve this goal compared to 40 C57BL/6 mice. Although oocyst yields per mouse are similar for both mouse strains, the survival rate for CF-1 mice is higher, resulting in reduced lost production time per study when compared to the C57BL/6 mice. This study presents a more efficient and cost effective dexamethasone suppressed murine model of propagating high concentrations of C. parvum oocysts.
The overall objective of this task is the development of improved occurrence detection methods for protozoan parasites and Microsporidia. Since this work is a primary focus of the Branch, this task supports several individual projects related to sample preparation and protozoan detection. Together these projects will lead to complete methods able to support the UCMR and the CCL2 and CCL3.
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Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice (PDF,NA pp, 23 KB, about PDF)
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LAB
MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION
BIOHAZARD ASSESSMENT RESEARCH BRANCH