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I. Effects of a Dopamine Receptor Antagonist on Fathead Minnow, Pimephales promelas ,Reproduction
VILLENEUVE, DAN, N. GARCIA-REYERO, D. MARTINOVIC, N. D. MUELLER, J. E. CAVALLIN, E. J. DURHAN, E. A. MAKYNEN, K. M. JENSEN, M. D. KAHL, L. S. BLAKE, E. J. PERKINS, AND G. T. ANKLEY. I. Effects of a Dopamine Receptor Antagonist on Fathead Minnow, Pimephales promelas ,Reproduction . ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. Elsevier Science Ltd, New York, NY, 73(4):472-477, (2010).
Results suggest that non-lethal concentrations of haloperidol do not directly impair fish reproduction or induce ovarian molecular responses that could serve as biomarkers of exposure to D2R antagonists.
This study used a 21 d fathead minnow (Pimephales promelas) reproduction assay to test the hypothesis that exposure to the dopamine 2 receptor (D2R) antagonist, haloperidol, would impair fish reproduction. Additionally, a 96 h experiment with fathead minnows and zebrafish (Danio rerio) was used to investigate haloperidol’s behavioral effects and compare effects on ovarian transcriptomes of these species using oligonucleotide microarrays. Continuous exposure to up to 20 ìg haloperidol/L had no significant effects on fathead minnow fecundity, secondary sex characteristics, gonad histology, or plasma steroid and vitellogenin concentrations. Male fathead minnows exposed to 50 ìg/L for 96 h were significantly more dominant than control males. Overall there was little similarity in the identity and functions of genes differentially expressed in fathead minnows versus zebrafish. Results suggest that non-lethal concentrations of haloperidol do not directly impair fish reproduction or induce ovarian molecular responses that could serve as biomarkers of exposure to D2R antagonists.
URLs/Downloads:Effects of a Dopamine Receptor Antagonist on Reproduction, Behavior, and Gene Expression in Cyprinid Fish Exit
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
MID-CONTINENT ECOLOGY DIVISION
TOXIC EFFECTS CHARACTERIZATION RESEARCH