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Identification of gene biomarkers for respiratory synctial virus infection in a bronchical epithelial cell line
Citation:
HUANG, Y. T., L. Zhuowei, H. Xhevahire, R. B. DEVLIN, M. T. SCHMITT, E. Karoly, AND J. M. SOUKUP. Identification of gene biomarkers for respiratory synctial virus infection in a bronchical epithelial cell line. GENOMICS. Academic Press Incorporated, Orlando, FL, 3(4):1113-125, (2009).
Impact/Purpose:
research resutls
Description:
Abstract: Respiratory syncytial virus (RSV) infection involves complex virus-host interplay. In this study, we analyzed gene expression in RSV-infected BEAS-2B cells to discover novel signaling pathways and biomarkers. We hybridized RNAs from RSV- or vehicle-treated BEAS-2B to Affymetrix HU133 plus 2.0 microarrays (n=4). At 4 and 24 hours post-infection, 277 and 900 genes (RSV/control ratio ≥2.0 or ≤0.5), and 1 and 12 pathways respectively were significantly altered. Twenty-three and 92 genes at 4 and 24 hours respectively matched respiratory disease biomarkers with ARG2 flagged at 24 hours and SCNN1G, EPB41L4B, CSF1, PTEN, TUBB1 and ESR2 at both time points. Hierachical clustering showed a cluster containing ARG2 and IL8. In human bronchial epithelial cells, RSV upregulated arginase II protein. Knockdown of ARG2 increased RSV-induced IL-8, LDH and histone release. With microarray, we identified novel proximal airway epithelial cell genes that may be tested in the sputum samples as biomarkers of RSV infection.
