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Concentrating Toxoplasma gondii and Cyclospora cayetanensis from surfacewater and drinking water by continuous separation channel centrifugation
Citation:
Borchardt, M. A., S. K. spencer, P. D. Bertz, M. W. WARE, AND H. LINDQUIST. Concentrating Toxoplasma gondii and Cyclospora cayetanensis from surfacewater and drinking water by continuous separation channel centrifugation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, 107(4):1089-97, (2009).
Impact/Purpose:
- evaluates the effectiveness of continuous separation channel configuration for concentrating Toxoplasma gondii and Cyclospora cayetanensis from drinking water and environmental waters. This research concluded that T.gondii and C.cayetanensis are effectively concentrated from various water matrices by continuous separation channel centrifugation. Waterborne transmission of T. gondii and C. cayetanensis presents another challengein producting clean drinking water and protecting public health. Detection of these parasites relies on effectively concentrating ooncysts from ambient water, otherwise false negatives may result. Validation data specific to T. gondii and C. cayetanensis concentration methods are limited. Continuous separation channel centrifugation recovers oocysts with high efficiency and precision, the method attributes required for accurately assessing the risk of waterborne transmissions
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Concentrating Toxoplasma gondii and Cyclospora cayetanensis from surfacewater and drinking water by continuous separation channel centrifugation