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Real-Time qPCR Data Analysis and Determination of Method Performance
Citation:
HAUGLAND, R. A. Real-Time qPCR Data Analysis and Determination of Method Performance. Presented at WQTC Conference Workshop, Cincinnati, OH, November 12 - 16, 2008.
Impact/Purpose:
1. Determine effects of storage by freezing of NEEAR study samples on QPCR measurements of enterococci. 2. Identify, from an expanded pool of candidates, the general fecal indicator microorganism(s) (i.e. different taxonomic or phylogenetically-related groups from all animal sources) whose densities in NEEAR study water samples, as measured by QPCR analysis, best correlate with illness rates monitored in the NEEAR study. 3. Determine whether illnesses rates in the NEEAR studies are better correlated with QPCR measurements of fecal indicator organisms from human as opposed to general sources (subject to the availability or development of suitable QPCR assays for human fecal indicators).
Description:
Quantitative PCR for the Water Mmicrobiologist course material to be presented at the WQTC Conference Workshop, Cincinnati, OH, November 16, 2008