You are here:
PROTEOMIC PROFILING OF CULTURED HUMAN BLADDER CELLS AFTER TRIVALENT ARSENICAL EXPOSURES (SOT 2008)
ORTIZ, P. A., K. WALLACE, AND W. M. WINNIK. PROTEOMIC PROFILING OF CULTURED HUMAN BLADDER CELLS AFTER TRIVALENT ARSENICAL EXPOSURES (SOT 2008). Presented at Society of Toxicology Annual Meeting, Seattle, WA, March 16 - 20, 2008.
Chronic exposure to arsenic has been associated with human cancers of the bladder, kidney, lung, liver, and skin. Inorganic arsenic is biotransformed in a stepwise manner via both a reduction and then an oxidative methylation step in which arsenic cycles between +5 and +3 oxidation states. To identify global protein-level changes associated with exposure to the individual trivalent arsenicals, a comparative proteome analysis of human urothelial cells (UROtsa) has been performed. UROtsa cells have been shown not to biotransform arsenic, making them a good model system for study. UROtsa cells treated with 1 μM of arsenite (AsIII), monomethylarsonous acid (MMAIII) and dimethylarsinous acid (DMAIII) for 24h were examined by a 2D difference gel electrophoresis (DIGE) approach and proteins were identified by nanospray-liquid chromatography-tandem mass spectrometry (LC-MSMS) analysis. Exposure to MMAIII showed the most significant changes in the protein expression profiles, exhibiting 64 differentially expressed proteins, including thioredoxin reductase, transgelin, aconitase, cofilin and glyceraldehyde-3-phosphate dehydrogenase. Western blots of a subset of these proteins are being performed to validate the LC-MSMS results. Understanding the effects of these trivalent arsenicals on protein expression may provide information crucial to the understanding of key molecular events underlying their toxicity.
To identify global protein-level changes associated with exposure to the individual trivalent arsenicals, a comparative proteome analysis of human urothelial cells (UROtsa) has been performed.
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
ENVIRONMENTAL CARCINOGENESIS DIVISION