Keywords:
WATER, HUMAN ENTERIC VIRUSES, COXSACKIEVIRUS, ECHOVIRUS, EMERGING PATHOGENS, CCL,
Project Information:
Progress
:A method to remove inhibitors of the Reverse Transcription-Polymerase Chain Reaction was developed. The method involves extraction of the virus containing sample with a chemical/solvent mixture. The effectiveness of the method was demonstrated in a cooperative study with the U.S. Geological Survey using five NAQWA sites.
A draft method on the detection of coliphages for a future edition of Standard Methods for the Examination of Water and Wastewater was completed, reviewed by the Joint Task Group and revised. The method includes the following protocols: somatic coliphage assay, male-specific coliphage assay using Escherichia coli Famp, male-specific coliphage assay using Salmonella typhimurium WG49, single agar layer method, and membrane filter method for male-specific coliphages.
Oligonucleotide primers for hepatitis E and group B rotaviruses were designed, synthesized and evaluated.
Key parameters of the common virus concentration, elution and concentration techniques were optimized under a cooperative agreement with the University of Cincinnati.
Reports:
Hurst, C. J., and Lindquist, H.D.A. 2000. Defining the ecology of viruses. In: Viral Ecology, C.J. Hurst (Ed.), San Diego, California: Academic Press
Fout, G.S. 1999. Method for the recovery and assay of total culturable viruses from sludge. In Control of pathogens and vector attraction in sewage sludge. U.S. Environmental Protection Agency, Washington D.C., Publication No. EPA/625/R-92/013 (Oct. 99 rev.).
Presentation:
Thompson, S., Jackson, J.L., Suva-Castillo, M., Yanko, W.A., and Williams, Jr., F.P. Detection of infectious adenovirus in tertiary treated and uv disinfected wastewater during a uv disinfection pilot study. Water Environment Federation's Annual Conference, Anaheim, California, October 14-18, 2000.
Grimm, A.C., and Fout, G.S. Development of a molecular method to identify hepatitis E virus. American Society for Virology, Ft. Collins, CO, July 8-12, 2000.
Fout, G.S. Recovery and Assay of Total Culturable Viruses from Biosolids. National Biosolids Conference/Workshop, June 26-29, 2000, Cincinnati, OH.
Relevance
:The Office of Research and Development and the Office of Water jointly published the Research Plan for Microbial Pathogens and Disinfection By-Products in Drinking Water in 1997. The support functions and methods being developed under this task relate to the plan's priority areas, "methods for detecting and enumerating Cryptosporidium and Giardia (including identifying the viability potential) in source and finished drinking water, and methods for other emerging protozoa," "methods for detecting and enumerating viruses in source and finished drinking waters" and "occurrence of and exposure to primary and opportunistic pathogens in distribution systems." Specifically, research is being performed in exposure research tasks EX.M.8, "application of PCR technologies and gene probes for virus detection in water" and EX.M.10, "methods for emerging viruses" and support is being provided to EX.M.7, "new protozoa agents," EX.M.9, "Norwalk virus" and "Ex.M.16, "PCR method for Legionella."
The Safe Drinking Water Act (SDWA), as amended in 1996, requires the Agency to establish criteria for a monitoring program for unregulated contaminants and to publish a list of contaminants to be monitored. The Office of Water has established a Contaminant Candidate List (CCL) that identifies agents being considered for monitoring under this rule. This list includes adenoviruses, caliciviruses, coxsackieviruses and echoviruses and microsporidia. Research on coxsackieviruses and echoviruses is being performed and support is being given to research on microsporidia under this task.
Methods developed may be used in future changes to the Ground Water Rule
Clients
:Office of Ground Water and Drinking Water (Dr. Paul Berger)
Research Component
:M/DBP (MICROBIAL)
Risk Paradigm
:EXPOSURE
Progress
:A method to remove inhibitors of the Reverse Transcription-Polymerase Chain Reaction was developed. The method involves extraction of the virus containing sample with a chemical/solvent mixture. The effectiveness of the method was demonstrated in a cooperative study with the U.S. Geological Survey using five NAQWA sites.
A draft method on the detection of coliphages for a future edition of Standard Methods for the Examination of Water and Wastewater was completed, reviewed by the Joint Task Group and revised. The method includes the following protocols: somatic coliphage assay, male-specific coliphage assay using Escherichia coli Famp, male-specific coliphage assay using Salmonella typhimurium WG49, single agar layer method, and membrane filter method for male-specific coliphages.
Oligonucleotide primers for hepatitis E and group B rotaviruses were designed, synthesized and evaluated.
Key parameters of the common virus concentration, elution and concentration techniques were optimized under a cooperative agreement with the University of Cincinnati.
Reports:
Hurst, C. J., and Lindquist, H.D.A. 2000. Defining the ecology of viruses. In: Viral Ecology, C.J. Hurst (Ed.), San Diego, California: Academic Press
Fout, G.S. 1999. Method for the recovery and assay of total culturable viruses from sludge. In Control of pathogens and vector attraction in sewage sludge. U.S. Environmental Protection Agency, Washington D.C., Publication No. EPA/625/R-92/013 (Oct. 99 rev.).
Presentation:
Thompson, S., Jackson, J.L., Suva-Castillo, M., Yanko, W.A., and Williams, Jr., F.P. Detection of infectious adenovirus in tertiary treated and uv disinfected wastewater during a uv disinfection pilot study. Water Environment Federation's Annual Conference, Anaheim, California, October 14-18, 2000.
Grimm, A.C., and Fout, G.S. Development of a molecular method to identify hepatitis E virus. American Society for Virology, Ft. Collins, CO, July 8-12, 2000.
Fout, G.S. Recovery and Assay of Total Culturable Viruses from Biosolids. National Biosolids Conference/Workshop, June 26-29, 2000, Cincinnati, OH.
Relevance
:The Office of Research and Development and the Office of Water jointly published the Research Plan for Microbial Pathogens and Disinfection By-Products in Drinking Water in 1997. The support functions and methods being developed under this task relate to the plan's priority areas, "methods for detecting and enumerating Cryptosporidium and Giardia (including identifying the viability potential) in source and finished drinking water, and methods for other emerging protozoa," "methods for detecting and enumerating viruses in source and finished drinking waters" and "occurrence of and exposure to primary and opportunistic pathogens in distribution systems." Specifically, research is being performed in exposure research tasks EX.M.8, "application of PCR technologies and gene probes for virus detection in water" and EX.M.10, "methods for emerging viruses" and support is being provided to EX.M.7, "new protozoa agents," EX.M.9, "Norwalk virus" and "Ex.M.16, "PCR method for Legionella."
The Safe Drinking Water Act (SDWA), as amended in 1996, requires the Agency to establish criteria for a monitoring program for unregulated contaminants and to publish a list of contaminants to be monitored. The Office of Water has established a Contaminant Candidate List (CCL) that identifies agents being considered for monitoring under this rule. This list includes adenoviruses, caliciviruses, coxsackieviruses and echoviruses and microsporidia. Research on coxsackieviruses and echoviruses is being performed and support is being given to research on microsporidia under this task.
Methods developed may be used in future changes to the Ground Water Rule
Clients
:Office of Ground Water and Drinking Water (Dr. Paul Berger)
Research Component
:CCL (MICROBIAL)
Risk Paradigm
:EXPOSURE
Project IDs:
ID Code
:EX.M.8 and EX.M.10
Project type
:ORD-DW Plan
ID Code
:5423
Project type
:OMIS