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POLYMORPHIC MICROSATELLITE LOCI FROM NORTHERN AND MEXICAN CORN ROOTWORMS (INSECTA: COLEOPTERA: CHRYSOMELIDAE) AND CROSS-AMPLIFICATION WITH OTHER DIABROTICA SPP
Citation:
WAITS, E. R. AND U. STOLZ. POLYMORPHIC MICROSATELLITE LOCI FROM NORTHERN AND MEXICAN CORN ROOTWORMS (INSECTA: COLEOPTERA: CHRYSOMELIDAE) AND CROSS-AMPLIFICATION WITH OTHER DIABROTICA SPP. MOLECULAR ECOLOGY NOTES. Blackwell Publishing, Malden, MA, 8:707-709, (2008).
Impact/Purpose:
The objective of this task is to develop genetic methodologies for evaluating and decreasing ecological risks posed by genetically modified crops (GPRA goal 4.5.2). This research has the following primary aims:
measurement of population genetic parameters for target pest populations in order to inform pest resistance management models genetic analysis of resistance/tolerance to Bt-toxin in laboratory pest strains to investigate the genetic basis of Bt resistance development of molecular genetic indicators of exposure of nontarget organisms to Bt toxins development and evaluation of a genetic monitoring program for establishing population trends and probable impacts for non-target populations near GM-crop fields.
Description:
The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we present fourteen polymorphic microsatellite loci isolated from libraries constructed using pooled northern and Mexican corn rootworm genomic DNA. Polymorphism in other Diabrotica, including the banded cucumber beetle, southern corn rootworm and western corn rootworm, is described.