Citation:

HORAN, K. L., R. FREEMAN, K. WEIGEL, M. SEMRET, S. L. PFALLER, T. C. COVERT, D. V. SOOLINGEN, S. C. LEAO, M. A. BEHR, AND G. A. CANGELOSI. ISOLATION OF THE GENOME SEQUENCE STRAIN MYCOBACTERIUM AVIUM 104 FROM MULTIPLE PATIENTS OVER A 17-YEAR PERIOD. JOURNAL OF CLINICAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 44(3):783-789, (2006).

Impact/Purpose:

Develop a real-time PCR method for the rapid detection and quantification of Mycobacterium avium and M. intracellulare in drinking water.

Determine the subspecies of those M. avium complex bacteria in EPA culture collection.

Description:

The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated form an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high throughput, large sequence polymorphism (LSP) based genotyping test, in which DNA form each strain was tested by PCR for the presence or absence of 4 hypervarible genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. this subset of 19 isolates was then to a high resolution repetitive sequence based PCR typing which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States and they were isolated over a 17 year time span. Therefore the sequenced genome of M. avium, strain has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity these observations also show that strains of the pathogen can be genotypically stable over extended time periods.

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