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An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method
Citation:
VARUGHESE, E., L. J. WYMER, AND R. A. HAUGLAND. An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 71(1):66-70, (2007).
Impact/Purpose:
Using robotics and the most probable number technique, a 96-microwell method was developed to compare two procedures for the enumeration of viable B. globigii spores subjected to chlorine treatment: broth-culture enrichment followed by real-time polymerase chain reaction (PCR) analysis; and filter-plating on agar media. Recoveries of chlorine-treated spores were improved by broth-enrichment over filter-plating whereas recoveries of non-treated spores were not significantly different in the two procedures.
Description:
Journal article
