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LIBRARY-DEPENDENT MICROBIAL SOURCE TRACKING OF ENTEROCOCCUS SP. USING AFLP AND BOX-PCR
Citation:
BUDINOFF, C. R., J. D. WILLIS, AND M. MOLINA. LIBRARY-DEPENDENT MICROBIAL SOURCE TRACKING OF ENTEROCOCCUS SP. USING AFLP AND BOX-PCR . Presented at American Society for Microbiology Annual Meeting, Toronto, ON, CANADA, May 21 - 25, 2007.
Impact/Purpose:
The objective of the proposed study is to evaluate and apply fast and reproducible DNA-based technology that can detect and track fecal contamination back to its source in complex environmental matrices, including recreational and drinking water resources.
Description:
Library-dependent microbial source tracking (LD MST) methods are one of the approaches used to identify nonpoint sources of fecal contamination in support of total maximum daily load implementation. However, LD MST methods have been questioned due to the high temporal and spatial variability of selected genotypes. Our goal was to examine the temporal variability of Enterococcus sp. isolates through the application of two genotyping LD MST methods, amplified fragment length polymorphism (AFLP) and repetitive polymerase chain reaction with BOX-primers (BOX-PCR). The library consists of 1600 isolates collected over a seasonal cycle at two separate bovine farms. Samples were collected from pre-farm sites (non-impacted), farm sites (impacted), and fecal matter.