Citation:

SAXENA, R. K., W. C. WILLIAMS, AND M. I. GILMOUR. SUPPRESSION OF BASAL AND CYTOKINE INDUCED EXPRESSION OF ANTIGEN PRESENTATION MARKERS ON MOUSE LUNG EPITHELIAL CELLS EXPOSED TO DIESEL EXHAUST PARTICLES. . American Journal of Biochemistry and Biotechnology. Science Publications, New York, NY, 3(4):187-192, (2007).

Impact/Purpose:

we examined the effect of in vitro exposure to DEP on basal and cytokine induced expression levels

Description:

Diesel exhaust particles (DEP) constitute a significant component of airborne particulates in urban environment. Exposure to DEP is known to enhance susceptibility to viral and bacterial infections. We hypothesized that DEP could partially exert its effect on disease susceptibility by interfering with antigen presentation required for the generation of protective adaptive immune responses. In the present study we examined the effect of in vitro exposure to DEP on basal and cytokine induced expression levels of certain molecules [MHC class I and class II molecules, ICAM1 (CD54) and B7.1 (CD80)] on mouse lung epithelial cells that are intimately involved with the presentation of viral and bacterial antigens to T cells. LA4 and TC1 mouse epithelial cell lines were activated with a mixture of cytokines (cytokine mixture, CM comprising 10 ng/ml each of mouse recombinant IFNγ, TNFα and IL1β) in the presence or absence of DEP. CM significantly boosted the expression of MHC I and ICAM 1 antigens on epithelial cells. Exposure to DEP significantly inhibited the basal and CM induced expression of MHC I and ICAM 1 expression, but did not interfere with the constitutive expression of B7.1 on epithelial cells. Significant levels of MHC II antigens were not expressed on the epithelial cells indicating that they may not be involved with the presentation of exogenous antigens. These results suggest that DEP may interfere with the cellular machinery involved with the presentation of viral antigens.

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