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PROTEOMIC PROFILING OF CULTURED HUMAN BLADDER CELLS AFTER TRIVALENT ARSENICAL EXPOSURES
Citation:
ORTIZ, P. A., K. WALLACE, AND W. M. WINNIK. PROTEOMIC PROFILING OF CULTURED HUMAN BLADDER CELLS AFTER TRIVALENT ARSENICAL EXPOSURES. Presented at Experimental Biology 2007, Washington, DC, April 28 - May 02, 2007.
Description:
Chronic exposure to arsenic has been associated with human cancers of the bladder, kidney, lung, liver, and skin. Inorganic arsenic is biotransformed in a stepwise manner via both a reduction and then an oxidative methylation step in which arsenic cycles between +5 and +3 oxidation states. To monitor protein-level changes associated with exposure to the individual trivalent arsenicals, a proteomic analysis of human urothelial cells (UROtsa) has been performed. UROtsa cells have been shown to only slightly biotransform arsenic, making them a good model system for study. Arsenite (AsIII), methylarsonous acid (MMAIII ) and dimethylarsinous acid (DMAIII) treated UROtsa cells are being examined by 2D difference gel electrophoresis (DIGE) at a 3-11 pH range. Thirty two protein spots changed in intensity after AsIII exposure. These proteins have been identified by LC-MS/MS. These identified proteins participate in
antigen processing, metal ion binding, protein folding, apoptosis regulation, biopolymer metabolism and oxidative stress response. We are continuing to analyze the effects of the MMAIII and DMAIII exposure on protein expression. Understanding the effects on protein expression induced by each of the three individual trivalent arsenicals may provide information crucial to understanding the key molecular events underlying their toxicity.
This is abstract does not necessarily reflect EPA policy.