You are here:
ELISA AND SOL-GEL BASED IMMUNOAFFINITY PURIFICATION OF THE PYRETHROID BIOALLETHRIN IN FOOD AND ENVIRONMENTAL SAMPLES
KAWARE, M., A. BRONSHTEIN, J. SAFI, J. M. VAN EMON, J. C. CHUANG, B. HOCK, K. KRAMER, AND M. ALTSTEIN. ELISA AND SOL-GEL BASED IMMUNOAFFINITY PURIFICATION OF THE PYRETHROID BIOALLETHRIN IN FOOD AND ENVIRONMENTAL SAMPLES. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. American Chemical Society, Washington, DC, 54:6482-6492, (2006).
The overall objectives of the task include several components: (1) develop immunochemical methods for compounds difficult to analyze by conventional methodologies; (2) tailor immunochemical methods to support specific human exposure assessment studies; (3) team immunochemical sample preparations with instrumental analysis such as mass spectrometry for in-depth sample characterization; (4) provide methods to support NERL's human exposure and environmental monitoring efforts; (5) provide analytical methods that improve risk assessments by reducing the amount of uncertainity in environmental measurements; (6) provide multimedia analytical methods to support an integrated multimedia approach to assess and characterize risk to human health and the environment; (7) provide sponsorship of annual immunochemistry research meetings as a forum for stimulating interest and discussion on current or emerging bioanalytical methods; (8) develop and incorporate rapid, cost-effective laboratory and field portable immunochemical techniques such as enzyme-linked immunosorbent assay (ELISA) methods into monitoring studies and human exposure field surveys to delineate sub-populations of "highly exposed" individuals for detailed follow-up studies.
Specific method needs have been identified through consultations with client office personnel. This Task strives to fulfill those needs as appropriate. In particular, methods for pyrethroids, e.g., permethrin, cypermethrin, and deltamethrin are being developed and evaluated. Efficient sample preparations are under development for exposure samples using pressurized liquid extraction. Confirmation will be achieved using high performance liquid chromatography (HPLC). A rapid immunoassay approach for the analysis of 2,4-D in urine will be completed and a SOP and report written. Immunoaffinity chromatography sample preparations for the pyrethroids will be developed. Work will continue on the application of antibody replacements such as molecularly imprinted polymers. Additional candidate analytes will be identified for a tiered approach and to guide development of the next Task. Flexibility will be maintained to address methods and measurement issues as they arise during the task period which ends in FY06. The objective of the Task is to develop bioanalytical methods to support exposure monitoring studies during the task period.
The peer-reviewed article describes the development of a new sol-gel based immunoaffinity purification procedure and an immunoassay for the pyrethroid bioallethrin. The immunoaffinity chromatography procedure was applied to food samples providing an efficient cleanup prior to immunoassay or gas chromatography-mass spectrometry (GC-MS) detection. The immunoaffinity column removed interfering components in the acetone extracts of the food samples as detected by GC-MS and yielded high recovery of bioallethrin from spiked extracts. Environmental soil and dust samples could be analyzed by an enzyme-linked immunosorbent assay (ELISA) without a cleanup step.