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BRAIN MICROGLIA (BV2) RESPONSE TO NON-PHOTOACTIVATED TIO2 NANOPARTICLES: IMPLICATIONS FOR NANOPARTICLE NEUROTOXICITY.
Citation:
LOWRY, G. V., T. LONG, N. SALEH, T. PHENRAT, AND B. VERONESI. BRAIN MICROGLIA (BV2) RESPONSE TO NON-PHOTOACTIVATED TIO2 NANOPARTICLES: IMPLICATIONS FOR NANOPARTICLE NEUROTOXICITY. Presented at American Chemical Society, Denver, CO, June 18 - 22, 2006.
Description:
Engineered nanoparticles are attractive for use in medical, industrial, and military sectors, but little is known of their interactions with biological systems. Recent studies indicate that some are not completely benign to biological and environmental targets. Here, the response of brain macrophages (BV2) to nanosized TiO2 (Degussa P25) was evaluated. Degussa P25 was extensively characterized in media and physiological buffer which both have high ionic strength (160mM) and high concentrations of Mg2+ and Ca2+. BV2 were exposed to non-cytotoxic doses of TiO2- in the dark, and the immediate and prolonged release of ROS from the macrophages was measured over a 120 min exposure period using various fluoroprobes. TEM was used to document the phagocytic response of the microglia to P25 exposure. These data demonstrate that non-photoactivated nanosize TiO2 stimulate microglia to produce ROS through the oxidative burst and interference with mitochondrial ETC. The sustained ROS generation by microglia suggests that neuronal damage may occur via an indirect pathway. (This abstract has been reviewed by the USEPA, NHEERL and does not necessarily reflect its policy).