You are here:
USE OF qRTPCR TO IDENTIFY POTENTIAL BIOMARKERS OF BROMATE EXPOSURE IN F344 MALE RAT KIDNEYS
Citation:
KNAPP, G. W., D. GETER, W. O. WARD, A. B. DEANGELO, R. D. OWEN, J. W. ALLEN, AND D. A. DELKER. USE OF qRTPCR TO IDENTIFY POTENTIAL BIOMARKERS OF BROMATE EXPOSURE IN F344 MALE RAT KIDNEYS. Presented at Second Annual Quantitative PCR Meeting, San Diego, CA, March 19 - 21, 2006.
Description:
Potassium bromate (KBrO3) is a drinking water disinfection by-product that is nephrotoxic and carcinogenic. To identify potential biomarkers of carcinogenicity, male F344 rats were chronically exposed to a carcinogenic dose (400mg/l) of KBrO3 in their drinking water. Kidneys were harvested and frozen after 52 and 100 weeks of chronic exposure. Gene expression profiles were generated on the kidney samples using Affymetrix microarrays. Nine significantly altered genes were identified with comparable expression patterns between the two exposure groups and deemed biologically relevant based on pathway analysis. Expression changes for the nine potential biomarkers were verified with quantitative reverse-transcriptase polymerase chain reaction (qRTPCR). Of these biomarker genes, the transcript most significantly changed was pendrin (S1c26a4), a solute carrier protein important in iodide uptake in the kidney and thyroid. The other potential biomarker genes include Aldh1a1, Aqp2, Calb1, Ccng1, Fabp5, Klk7, Ngfg and Slc12a1. The qRTPCR data confirmed the array results for all nine potential biomarkers from the 52 kidney samples. Future qRTPCR experiments are planned to evaluate gene expression changes at additional time points and dose levels to assess the reliability of the interrogated genes as biomarkers for kidney carcinogenesis.