You are here:
MICROARRAY ANALYSIS OF PM-INDUCED GENEEXPRESSION IN HUMAN BRONCHIAL EPITHELIAL CELLS
Citation:
SCHMITT, M. T., L. A. DAILEY, D. GRAFF, AND R. B. DEVLIN. MICROARRAY ANALYSIS OF PM-INDUCED GENEEXPRESSION IN HUMAN BRONCHIAL EPITHELIAL CELLS. Presented at Society of Toxicology Annual Meeting, San Diego, CA, March 05 - 09, 2006.
Description:
Ambient air particles (PM) are generally classified into 3 sizes; coarse (2.5, 10m), fine (0.1, 2.5m), and ultrafine (<0.lpm). Each particle size is evolved from different sources and transformation processes (e.g., combustion vs. mechanical abrasion, and atmospheric conversion and transformation vs. direct release) and their chemical compositions are quite different. The relative role each size fraction of PM plays in causing adverse health effects is still not clear at this time. The purpose of this study was to characterize global gene expression and cellular pathways associated with each of the PM size fractions and to potentially identify biomarkers of exposure or effect for individual PM components. Ambient PM was collected in 3 different size fractions from Chapel Hill air, particles were extracted from foam or filter matrices and lyophilized. Human primary airway epithelial cells were exposed to 250g/ml for 6hrs. Human genome microarrays coupled with a commercially available software package were used for gene expression analysis. Expression patterns were statistically evaluated at 3 significance levels, p<0.05, p<0.01, and p<0.005 and differential expression examined at 1.5 fold change for each size fraction. There were 4851 genes that were significantly differentiated at p<0.05 with 470 that were differentially changed 1.5 fold over controls. Of these 470 differentially expressed genes 60, 154, and 100 were unique to the coarse, fine, and ultrafine PM fractions, respectively. Fine and ultrafine PM fractions differentially expressed 82 genes in common while coarse and ultrafine only shared 12 common genes. Current analysis is focused on evaluation of cellular pathways activated by each size fraction. Differentially expressed genes and pathways will also be compared with chemical components present in each of the size fractions. This abstract of a proposed presentation does not necessarily reflect EPA policy.