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ALDEHYDE DEHYDROGENASES EXPRESSION DURING POSTNATAL DEVELOPMENT: LIVER VS. LUNG
Citation:
YOON, M., M. C. MADDEN, AND H. A. BARTON. ALDEHYDE DEHYDROGENASES EXPRESSION DURING POSTNATAL DEVELOPMENT: LIVER VS. LUNG. Presented at Society of Toxicology Annual Meeting, San Diego, CA, March 05 - 09, 2006.
Description:
Aldehydes are highly reactive molecules present in the environment, and can be produced during biotransformation of xenobiotics. Although the lung can be a major target for aldehyde toxicity, development of aldehyde dehydrogenases (ALDHs), which detoxify aldehydes, in lung has been poorly studied. The expression of ALDHs in liver and lung across ages (postnatal days 1, 8, 22, and 60) was investigated in Wistar-Han rats using enzyme activity measurement and Western blotting. ALDHs activity was measured with 10mM propanal or 50pM hexanal in 1000xg supernatant (Si), mitochondria, cytosol, and microsomes from liver and lung. Total aldehyde oxidation capacity in liver develops with age, but stays constant in lung. In adult, the majority of hepatic ALDH activity was found in mitochondria while cytosolic ALDH activity was the highest contributor in lung. However, the relative contribution of each subcellular fraction within a tissue changed across ages suggesting an independent and a tissue-specific development of ALDH isoforms. Temporal differences in the development between ALDH1 and ALDH2 were investigated using Western blot analysis, which confirmed the relative expression of ALDH1 to ALDH2 changes according to developmental stages with a tissue specific manner. The overall developmental profiles of ALDH expression in a tissue appears to be determined by the different composition of ALDH isoforms within the tissue and their independent temporal and tissue-specific development indicating that in the case of aldehyde exposure, the in vivo responses may be tissue and age-dependent. (Supported by EPA-NRC #CR82879001. This abstract may not represent official EPA policy.)