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PREGNANCY LOSS ASSOCIATED WITH EXPOSURE TO PERFLUOROOCTANOIC ACID IN THE MOUSE
Citation:
LAU, C. S., J. R. THIBODEAUX, L. THAI, R. HANSON, M. R. BLANTON, E. S. HUNTER, B. D. ABBOTT, AND M. J. STRYNAR. PREGNANCY LOSS ASSOCIATED WITH EXPOSURE TO PERFLUOROOCTANOIC ACID IN THE MOUSE. Presented at 45th Annual Teratology Society Meeting, St Pete Beach, FL, June 25 - 30, 2005.
Impact/Purpose:
The objective of this task is to develop state-of-the-art methods for measuring xenobiotic compounds, to include the isolation of the analyte from the appropriate matrix (extraction), preconcentration (typically sorbent-based), and analysis via GC/MS and/or LC/MS. Once established, these methods will be applied in small scale pilot studies or demonstration projects. Particular emphasis will be placed on methods which are readily transferable to other laboratories, including those within the Human Exposure and Atmospheric Sciences Division (HEASD), the National Exposure Research Laboratory (NERL), other EPA Laboratories, Program Offices, Regions, and academic institutions.
Specific objectives of this task include the following:
1) Development of GC/MS and LC/MS methods for the measurement of key xenobiotic compounds and their metabolites (to include the pyrethroid pesticides, perfluorinated organic compounds, and the BFRs) in relevant environmental and biological matrices.
2) Development of efficient low cost methods for the extraction and clean up of these compounds collected from relevant matrices.
3) Determination of xenobiotic compound and metabolite concentrations in samples derived from laboratory and field monitoring studies to help assess exposures and evaluate associated risks.
Description:
Pregnancy loss in the mouse due to perfluorooctanoic acids (PFOA) was investigated in this present study. Daily administration of PFOA (20 or 40 mg/kg) by oral gavage to pregnant CD-1 mice from GD 1-17 led to 75% and 100% incidence respectively, of total resorption at term with corresponding maternal serum PFOA levels of 0.18 mg/ml and 0.27 mg/ml. In vitro mouse embryos exposed to PFOA greater than 0.4 mg/mL led to significant changes in embryo morphology with 1.25 mg/mL leading to 100 percent embryolethality.