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INACTIVATION OF HEPATITIS A VIRUS AND MS2 BY OZONE AND OZONE-HYDROGEN PEROXIDE IN BUFFERED WATER
Citation:
Hall, R. M. AND M. D. Sobsey. INACTIVATION OF HEPATITIS A VIRUS AND MS2 BY OZONE AND OZONE-HYDROGEN PEROXIDE IN BUFFERED WATER. WATER SCIENCE AND TECHNOLOGY. IWA Publishing, London, Uk, 27(3-4):371-378, (1993).
Description:
Disinfection of drinking water by chlorine is a primary means of preventing the transmission of waterborne disease, and its efficacy is well-established. The comparative inactivation of highly purified hepatitis A virus (HAV) and MS2 by 1 mg water/L, 2.0 and 0.4 mg ozone/L plus 0.6, 1.0 or 1.6 mg hydrogen peroxide/L was determined at temperatures from 3 to 10 C in 0.01 phosphate buffer (ph 6 to 10). Both HAV and MS2 were completely inactivated (3.9 to 6 log 10) within 5 seconds in ozone solutions. Hydrogen peroxide did not inactivate MS2, but did inactivate 95% of the HAV. Both HAV and MS2 were completely (3.3 to 5.5 log) inactivated within five seconds in the oxidant mixtures at pH 6 to pH 8, but MS2 survival exceeded that of HAV at pH 10. Different methods of ozone concentration measurement gave differing ozone residual values, which would affect the interpretation of viral disinfection studies. MS2 was found to be a good model for predicting HAV inactivation by ozone and mixtures of ozone and hydrogen peroxide when both viruses are purified in this manner. Further studies of virus disinfection by ozone and other oxidants should be made under highly controlled methods of virus purification, the timing of ozone residual determinations, and with ozone residual measurement methods that are specific for ozone.