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TECHNOLOGIES FOR MONITORING AND MEASUREMENT OF DIOXIN AND DIOXIN-LIKE COMPOUNDS IN SOIL AND SEDIMENT. HYBRIZYME CORPORATION AHRC PCR KIT
Citation:
DINDAL, A. AND S. BILLETS. TECHNOLOGIES FOR MONITORING AND MEASUREMENT OF DIOXIN AND DIOXIN-LIKE COMPOUNDS IN SOIL AND SEDIMENT. HYBRIZYME CORPORATION AHRC PCR KIT. U.S. Environmental Protection Agency, Washington, DC, EPA/540/R-05/005 (NTIS PB2005108390), 2005.
Impact/Purpose:
The objective of this program is to promote the acceptance and use of innovative field technologies by providing well-documented performance and cost data obtained from field demonstrations.
Description:
A demonstration of technologies for determining the presence of dioxin and dioxin-like compounds in soil and sediment was conducted under EPA's Superfund Innovative Technology Evaluation Program in Saginaw, Michigan in April 2004. This report describes the performance evaluation of Hybrizyme Corporation's AhRC PCR" Kit. This is a technology that reports the concentration of aryl hydrocarbon receptor (AhR) binding compounds in a sample, and the units are reported as Ah Receptor Binding Units (AhRBU). The test included an assessment of how well the technology's results compared to results generated by established laboratory methods using high-resolution mass spectrometry.
The Hybrizyme ranking of AhR compounds from low to high was consistent with the reference laboratory ranking for the environmental samples within a site 70-90% of the time. The technologys calculated estimated method detection limit was 71 AhRBU. The data generated and evaluated during this demonstration showed that the Hybrizyme technology could be used as an effective tool to rank sample concentrations from low to high AhR activity within a particular environmental site, particularly considering that the cost ($35,023 vs. $398,029) and the time (< 2 weeks vs. 8 months) to analyze the 209 demonstration samples was significantly less than that of the reference laboratory.