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CHEMICALLY ACTIVATED LUCIFASE GENE EXPRESSION (CALUX) CELL BIOASSAY ANALYSIS FOR THE ESTIMATION OF DIOXIN-LIKE ACTIVITIY: CRITICAL PARAMETERS OF THE CALUX PROCEDURE THAT IMPACT ASSAY RESULTS
Citation:
WINDAL, I., M. S. DENISON, L. S. BIRNBAUM, N. VAN WOUWE, W. BAEYENS, AND L. GOEYENS. CHEMICALLY ACTIVATED LUCIFASE GENE EXPRESSION (CALUX) CELL BIOASSAY ANALYSIS FOR THE ESTIMATION OF DIOXIN-LIKE ACTIVITIY: CRITICAL PARAMETERS OF THE CALUX PROCEDURE THAT IMPACT ASSAY RESULTS. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, 39(19):7357-7364, (2005).
Impact/Purpose:
To summarize information on the critical methodological parameters of the CALUX cell bioassay used for the screening and relative quantification of dioxins
Description:
The Chemically Activated Luciferase gene expression (CALUX) in vitro cell bioassay is an emerging bioanalytical tool that is increasingly being used for the screening and relative quantification of dioxins and dioxin-like compounds. Since CALUX analyses provide a biological response to all AhR active compounds present in a given sample extract containing a complex mixture of chemicals, interpretation of results is more complex than of chemical analyses. Operators in the laboratory can adjust many parameters when performing CALUX analyses and the applied procedure strongly affects the result and, hence, the interpretation of the results. This paper summarizes information on the critical methodological parameters of the CALUX bioassay which can affect the analyses. Moreover, the study aims to identify the ways that alteration of these parameters influences CALUX measurements. Attempts are also made to interpret the CALUX response.