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STATISTICAL EVALUATION OF AN ANALYTICAL GC/MS METHOD FOR THE DETERMINATION OF LONG CHAIN FATTY ACIDS
Citation:
CAMPO, P., G. A. SORIAL, M. T. SUIDAN, AND A. D. VENOSA. STATISTICAL EVALUATION OF AN ANALYTICAL GC/MS METHOD FOR THE DETERMINATION OF LONG CHAIN FATTY ACIDS. doi:10.1016/j.talant, G.D. Christian, J.-M. Kauffmann (ed.), TALANTA. Elsevier Science Ltd, New York, NY, 68(3):888-894, (2006).
Impact/Purpose:
To carry out a detailed evaluation of a chemical measurement process (CMP) for quantifying long chain fatty acids in water (C8 - C16).
Description:
In-depth evaluation of an analytical method to detect and quantify long chain fatty acids (C8 - C16) at trace level concentrations (25-1000 µg/l) is presented. The method requires derivatization of the acids with methanolic boron trifluoride, separation, and detection by gas chromatography-mass spectrometry. The calibration experiments passed all the tested performance criteria such as linearity, homoscedasticity, and ruggedness. The detection limits and related quantities were computed by applying the method detection limit, and the calibration line approximation. The values obtained by applying the latter approach were more reliable and consistent with the actual statistical theory of detection decisions and yielded the following concentrations: C8, 87.6 µg/l; C10, 45.2 µg/l; C11, 39.9 µg/l; C12, 37.7 µg/l; C14, 41.4 µg/l and C16, 40.6 µg/l. Two different gas-liquid chromatographic columns were tested and similar results achieved, which shows the ruggedness of the method.
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STATISTICAL EVALUATION OF AN ANALYTICAL GC/MS METHOD FOR THE DETERMINATION OF LONG CHAIN FATTY ACIDS