CHLORINE INACTIVATION OF BACTERIAL BIOTERRORISM AGENTS
Citation:
Rose, L. J., G Rice, B. Jensen, R. Murago, A. Peterson, R. Donlan, AND M. J. Arduino. CHLORINE INACTIVATION OF BACTERIAL BIOTERRORISM AGENTS. APPLIED ENVIRONMENTAL MICROBIOLOGY 71(1):566-568, (2005).
Impact/Purpose:
Seven species of bacteria from the Centers for Disease Control and Prevention's list of possible bioterrorism agents (A and B list) were tested for susceptiblity to free chlorine at pH 7. Yersinia pestis, Brucella suis, Brucella melitensis, Burkholderia pseudomallei, and Burkholderia mallei demonstrated 3 log reduction within 1 min when exposed to 0.5 ppm free available chlorine (FAC) at either 5C or 25C. Ct values (C = concentration of disinfectant in mg/L; t= time of cell contact with disinfectant) were calculated at 0.7 or less for a 3 log reduction. Francisella tularensis survived longer, with one isolate requiring 5 min of exposure to 0.7 mg/L FAC at 25C for 2.5 log inactivation. Ct values ranged from 1.0 for F. tularensis LVS at 25C to 10.3 for F. tularensis NY (* at 5C for a 3 log reduction. Bacillus anthracis spores were expectedly more resistant than the gram negative bacteria tested, with strain variability seen. Bacillus anthracis Sterne was inactivated by 4 logs after 2 hrs exposure to 1.0 mg/L FAC at 225 C, while Bacillus anthracis Ames was reduced by 2 logs after 2 hrs. exposure to 0.8 mg/L. Ct values for a 3 log reduction of B. anthracis Sterne were calculated to be 271 at 5C and 86 at 25C. B.anthracis Ames Ct values were 339 at 5C and 102 at 25C.
Description:
Journal
