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ROXARSONE AND TRANSFORMATION PRODUCTS IN CHICKEN MANURE: DETERMINATION BY CAPILLARY ELECTROPHORESIS-INDUCTIVELY COUPLES PLASMA MASS SPECTROMERTY
Citation:
Rosal, C G., G M. Momplaisir, AND E M. Heithmar. ROXARSONE AND TRANSFORMATION PRODUCTS IN CHICKEN MANURE: DETERMINATION BY CAPILLARY ELECTROPHORESIS-INDUCTIVELY COUPLES PLASMA MASS SPECTROMERTY. ELECTROPHORESIS. Wiley-VCH, Weinheim, Germany, 26(7-8):1606-1614, (2005).
Impact/Purpose:
The overall goals of the task are to apply NERL's core capability in advanced chemical science and technology for maximum benefit in estimating exposures of ecosystems and humans to chemical stressors and to identify emerging pollution concerns, in particular long-range airborne transport of contaminants. This task comprises several subtasks, each with individual objectives:
Subtask 1: screen exposures of National Park PRIMENet ecosystems to chemical stressors, identifying indications of exposure requiring further evaluation, and use these samples evaluate new analytical methods as replacements for standard methods in future assessments of ecosystem contaminant exposures.
Subtask 2: evaluate a new mercury analytical approach with superior performance on complex solid matrices such as biological tissues, and apply the approach to estimating exposure of ecosystems and humans to mercury.
Subtask 3: determine distribution patterns of chemical contaminants in the southern Sierra Nevada Range of California, investigate topographic and weather factors that may influence the distributions, and determine if a correlation exists between contaminant distributions and extirpation patterns of the mountain yellow-legged frog.
Subtask 4: provide analytical methods to measure a number of inorganic and organic arsenic species in a variety of environmental matrices, elucidate the environmental transformations undergone by organoarsenic animal-feed additives, and determine if the potential exists for substantially increased exposure of humans and aquatic organisms to arsenic.
Description:
The determination of the animal feed additive roxarsone (3-nitro-4- hydroxyphenylarsonic acid) and six of its possible transfonnation products (arsenite, arsenate, monomethylarsonate, dimethylarsinate, 3-amino-4-hydroxyphenylarsonic acid, and 4-hydroxyphenylarsonic acid) in chicken manure was investigated using capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICPMS). Initial method development was conducted using ultraviolet (UV) detection for ruggedness and time efficiency. Separation of these seven arsenic species was effected using a 20 mM phosphate buffer at pH 5.7. The CE-ICPMS limits of detection in terms of As for each of the species was in the low ugL-1 range, corresponding to absolute detection limits in the range 20-70 fg As (based on a 23-nL injection). Overall, the method developed in this study provides high selectivity and low limits of detection (1-3 ug L-1 or low-ppb, based on As), uses small sample volume (low nL), and produces minimal wastes.