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QUANTIFICATION OF 2,4-D ON SOLID-PHASE EXPOSURE SAMPLING MEDIA BY LC/MS/MS
Citation:
Gardner, M., M. SpruillMcComb, J. Beach, L. Michael, K W. Thomas, AND R. Helburn. QUANTIFICATION OF 2,4-D ON SOLID-PHASE EXPOSURE SAMPLING MEDIA BY LC/MS/MS. JOURNAL OF ANALYTICAL TOXICOLOGY. Preston Publications Incorporated, Niles, IL, 29(3):188-192, (2005).
Impact/Purpose:
The primary goal of the AHS Pesticide Exposure Study (AHS/PES) is to measure exposure to applied pesticides for a subset of the cohort of private pesticide applicators and to provide data to evaluate exposure algorithms developed for exposure classification in the study cohort.
Description:
Three types of solid phase chemical exposure sampling media: cellulose, polyurethane foam (PUF) and XAD-2, were analyzed for 2,4-D and the amine salts of 2,4-D. Individual samples were extracted into acidified methanol and the extracts were analyzed via LC/MS/MS using electrospray ionization and a C8 reversed-phase LC column. 13C 2,4-D and a labeled benzoic acid (13C or d5) were employed as the surrogate and internal standard respectively. Over a three year study of pesticide exposure among agricultural workers involving a high volume of analyses, values of average % recovery for 13C 2,4-D on personal exposure samples ranged from 87 to 98 with a % RSD ranging from 7 to 12. The performance of the method compares favorably with that of the current NIOSH recommended approach for the analysis of 2,4-D on these types of exposure sampling matrices.
The United States Environmental Protection Agency through its Office of Research and Development funded and managed the research described here under Contract 68-D-99-012 to RTI International. It has been subjected to Agency review, and approved for publication.