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DIRECT-DEPOSITION INFRARED SPECTROMETRY WITH GAS AND SUPERCRITICAL FLUID CHROMATOGRAPHY
Gurka, D., S. Pyle, R. Titus, AND E. Shafter. DIRECT-DEPOSITION INFRARED SPECTROMETRY WITH GAS AND SUPERCRITICAL FLUID CHROMATOGRAPHY. Analytical Chemistry 66(15):2521-2528, (1994).
A direct-deposition Fourier transform infrared (FT-IR) system has been evaluated for applicability to gas chromatography (GC) and supercritical fluid chromatography (SFC) of environmental analytes. A 100-um i.d. fused-silica transfer line was used for GC, and a 50-um transfer line with an integral restrictor was used for SFC. Minimum identifiable quantities for GC/FT-IR ranged from 0.5 to 2.0ng. This is over an order of magnitude better than those reported for light-pipe FT-IR by this laboratory. However, some of this sensitivity improvement can probably be attributed to the smaller i.d. GC column used in this work and the ability to program the direct-deposition sample plate, thereby compensating for changes in analyte elution volume across the GC temperature ramp, or to the smaller detector area used in this work relative to that used for the light-pipe project. Excellent SFC/FT-IR chromatography was obtained for poly(ethylene glycols) of average molecular weights 400, 600, 1000, and 1500. It was established that poly(ethylene glycol) 400 would not pass through a gas chromatography. Newer supercritical fluid chromatographs can attain almost twice the highest pressure used in this work, which should allow the separation of even higher molecular weight analytes.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT