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DETERMINATION OF CARBENDAZIM IN WATER BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY ON-LINE WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY OR MASS SPECTROMETRIC DETECTION
Thomas, D. H., V. Lopez-Avila, L. Betowski, AND J. Van Emon. DETERMINATION OF CARBENDAZIM IN WATER BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY ON-LINE WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY OR MASS SPECTROMETRIC DETECTION. Journal of Chromatography A 724(1-2):207-217, (1996).
An automated method for the determination of carbendazim in water that combines high-performance immunoaffinity chromatography (HPIAC), high-performance liquid chromatography (HPLC) in the reversed-phase mode, and detection by either UV-Vis diode array detector (DAD) spectroscopy or mass spectrometry (MS) is presented here. This method allows for the on-line extraction, preconcentration, and positive confirmation of carbendazim with a throughput of one sample analyzed every 10 min. The method requires minimal manual sample pretreatment, yet it is free from coextracted interferences that often occur in solid-phase extraction based on nonspecific sorbents. The linear range of the calibration curve for 200-ul injections of carbendazim is 0.025 to 100 ug/l for HPIAC-HPLC-MS and 0.075 to 100 ug/1 for HPIAC-HPLC-DAD. Because the calibration curve is mass-dependent, lower detection limits could be achieved by applying larger sample volumes to the HPIAC column. The within-day precision is + 4.5% for HPIAC-HPLC-MS and +16% for HPIAC-HPLC-DAD for samples containing 0.1 ug/1 carbendazim. The results from this method correlate well with results from an ELISA.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT