Science Inventory

SCREEN-PRINTED TYROSINASE-CONTAINING ELECTRODES FOR THE BIOSENSING OF ENZYME INHIBITORS

Citation:

Wang, J., V. B. Nascimento, S. A. Kane, K. Rogers, M. R. Smyth, AND L. Angnes. SCREEN-PRINTED TYROSINASE-CONTAINING ELECTRODES FOR THE BIOSENSING OF ENZYME INHIBITORS. Talanta 43(11):1903-1907, (1996).

Description:

Disposal amperometric inhibition biosensors have been microfabricated by screen printing a tyrosinase-containing carbon ink. The decrease in the substrate (catechol) steady-state current, caused by the addition of various pesticides and herbicides, offers convenient quantitation of micromolar levels of these pollutants. Unlike esterase-based disposable strips, the tyrosinase thick-film devices can be fabricated by incorporating the enzyme within the carbon ink, and do not require a prolonged incubation step in the presence of the inhibitor. The effect of experimental variables, such as the enzyme loading or substrate concentration, is assessed. Applicability to an untreated river water sample is illustrated. Such use of single-use devices for monitoring toxins addresses the problem of irreversible enzyme inhibition, and holds great promise for on-site field analysis.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 01/01/1996
Record Last Revised: 12/22/2005
Record ID: 8916

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT