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THE ISOLATION AND IDENTIFICATION OF MYCOBACTERIUM AVIUM COMPLEX (MAC) RECOVERED FROM LOS ANGELES POTABLE WATER, A POSSIBLE SOURCE OF INFECTION IN AIDS PATIENTS
Glover, N., A. Holtzman, T. Aronson, S. Froman, O. G. Berlin, P. Dominguez, K. A. Kunkel, G. Overturf, G. Stelma, C. Smith, AND M. Yakrus. THE ISOLATION AND IDENTIFICATION OF MYCOBACTERIUM AVIUM COMPLEX (MAC) RECOVERED FROM LOS ANGELES POTABLE WATER, A POSSIBLE SOURCE OF INFECTION IN AIDS PATIENTS. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 4(2):63-72, (1994).
Los Angeles water was investigated as a possible source of Mycobacterium avium complex (MAC) infection in patients with AIDS. MAC consists of M.avium (MA), M. intracellulare (MI) and Mycobacterium X (MX)(positive for MAC by DNA probe but not MA or MI). The study included 13 reservoirs supplying 90% of the water to the City of Los Angeles, ten major hospitals and 58 dwellings. Water samples were filtered through 0.45 um Millipore filters and decontaminated with either sodium hydroxide and/or oxalic acid and cetylpyridinium chloride. The decontaminated filters were placed on selective Middlebrook 7H10 medium and observed for growth at three and eight weeks. Coccobacillary acid-fast bacilli were subcultured; Tween and nitrate negative isolates were identified by DNA hybridization probes. Eight morphotypes were observed and nontuberculous mycobacteria (NTM) were recovered from 12 (92%) reservoirs, 54 (95%) homes, and ten (100%) hospitals. Twenty-six (34%) of the NTM were probe positive for MAC. MA was isolated from 70% of hospitals, 9% of dwellings, and 15% of reservoirs. Serotyping and multilocus enzyme electrophoresis results demonstrate a genetic relatedness between some MA water and MA clinical isolates.