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COMPARISON OF UV INACTIVATION OF SPORES OF THREE ENCEPHALITOZOON SPECIES WITH THAT OF SPORES OF TWO DNA REPAIR-DEFICIENT BACILLUS SUBTILIS BIODOSIMETRY STRAINS
Marshall, M. M., S Hayes*, J. Moffett, C. R. Sterling, AND W. L. Nicholson. COMPARISON OF UV INACTIVATION OF SPORES OF THREE ENCEPHALITOZOON SPECIES WITH THAT OF SPORES OF TWO DNA REPAIR-DEFICIENT BACILLUS SUBTILIS BIODOSIMETRY STRAINS. Ornston, L.N. (ed.), APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 69(1):683-685.
The sensitivity of three Encephalitozoon spp. to ultraviolet (UV) inactivation was determined. Encephalitozoon intestinalis is a contaminant listed on the USEPA's 1998 Contaminant Candidate List (CCL). Also, use of DNA repair deficient strains of Bacillus subtilis were evaluated for their usefulness as biodosimeters. E. intestinalis, E. cuniculi and E. hellem exhibited Log 10 reductions of 3.2 at 60, 140 and 190 J/cm 2, respectively. UV inactivation studies with one B. subtilis strain showed similar UV inactivation kinetics of the three Encephalitozoon spp. These findings suffest that the Encephalitozoon spp. are readily inactivated at low UV fluences and spores from genetically engineered B. subtilis strains can useful surrogates when evaluating UV reactor performance via biodosimetry.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL RISK MANAGEMENT RESEARCH LABORATORY
WATER SUPPLY AND WATER RESOURCES DIVISION
MICROBIAL CONTAMINANTS CONTROL BRANCH