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AEROBIC BIODEGRADATION OF GASOLINE OXYGENATES MTBE AND TBA
Wilson, G. J., A. P. Richter, M. T. Suidan, AND A D. Venosa*. AEROBIC BIODEGRADATION OF GASOLINE OXYGENATES MTBE AND TBA. Wilderer, P. (ed.), WATER SCIENCE AND TECHNOLOGY. IWA Publishing, London, Uk, 43(2):277-284, (2001).
MTBE degradation was investigated using a continuously stirred tank reactor (CSTR) with biomass retention (porous pot reactor) operated under aerobic conditions. MTBE was fed to the reactor at an influent concentration of 150 mg/l (1.70 mmol/l). A second identifical rector was operated as a control under the same conditions with the addition of 2.66 /l of sodium azide, to kill any biological activity. Restuls from these experiments suggest that biomass retention is critical to the gradation of MTBE. The rate of MTBE removal was shown to be related to the VSS concentration. MTBE removal oexceeded 99.99% when the VSS concentration in the reactor was over 600 mg/l. Results obtained from batch experiments conducted on mixed liquor samples from the porous pot reactor indicate that the individual rates of biodegradation of MTBe and TBA were higher for initial concentrations of 15 mg/l than for concentration of 5 mg/l. The presence of TBA at lower concentrations did not effect the rate of MTBe degradation, however, higher concentrations of TBA did reduce the rate of biodegradation of MTBE. Denaturing Gradient Gel Electrophoresis (DGGE) analysis reveals that the culture consisted of a community of bacterial organisms of about 6 species.