Science Inventory

PHOSPHOLIPIDS OF FIVE PSEUDOMONAD ARCHETYPES FOR DIFFERENT TOLUENE DEGRADATION PATHWAYS

Citation:

Fang, J. AND M. J. Barcelona. PHOSPHOLIPIDS OF FIVE PSEUDOMONAD ARCHETYPES FOR DIFFERENT TOLUENE DEGRADATION PATHWAYS. The Fifth International In Situ & On-Site Bioremediation Symposium, San Diego, CA, 4/19-22/99.

Description:

Liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS) was used to determine phospholipid profiles for five reference pseudomonad strains harboring distinct toluene catabolic pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PK01, and Pseudomonas mendocina KR1. The five pseudomonad strains contained a predominant pool of phosphatidylethanolamines. Other phospholipids identified include phosphatidylglycerol, phosphatidylserine, phosphatidylmethylethanolamine, and phosphatidyldimethylethanolamine. There is a clear separation in phospholipid profiles that allows for the differentiation between the Pseudomonas and Burkholderia genera. Factor analysis of the phospholipid profiles showed that B. cepacia G4, P. putida mt-2, and B. pickettii PK01 can be clearly separated, while P. putida F1 and P. mendocina KR1 were clustered as a group.

Record Details:

Record Type: DOCUMENT (PRESENTATION/PAPER)
Product Published Date: 04/19/1999
Record Last Revised: 06/21/2006
Record ID: 63805

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

SUBSURFACE PROTECTION AND REMEDIATION DIVISION

ECOSYSTEM & SUBSURFACE PROTECTION