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LOW PRESSURE ULTRAVIOLET STUDIES FOR INACTIVIATION OF GIARDIA MURIS CYSTS
Hayes*, S, C Barnes, E W. Rice, M W. Ware, AND F W. Schaefer. LOW PRESSURE ULTRAVIOLET STUDIES FOR INACTIVIATION OF GIARDIA MURIS CYSTS. Presented at First International Congress on Ultraviolet Technologies, Washington, DC, 6/14-16/2001.
This research was initiated to confirm and expand the current database for the inactiviation of Giardia spp. using ultraviolet (UV) radiation. The path taken was to confirm earlier UV research that used excystation as the indication of viability. In this study, an in vitro excystation assay was employed in parallel with an animal infectivity assay which was performed using female, age matched CF-1 mice. G. muris cysts were produced in CF-1 female mice. Cysts were separated from fecal material using sucrose flotation and were further purified using a Percoll density gradient. A low-pressure UV source was used in all experiments. For animal infectivity assays, freshly prepared cysts were diluted, placed into a continuously stirred Petri dish, and exposed to UV irradiation using a collimated beam apparatus. Unexposed controls were included in each experiment.