Science Inventory

Anaerobic Biodegradation of Soybean Biodiesel and Diesel Blends under Methanogenic Conditions


Wu, S., M. Yassine, M. Suidan, AND A. Venosa. Anaerobic Biodegradation of Soybean Biodiesel and Diesel Blends under Methanogenic Conditions. Mark van Loosdrecht (ed.), WATER RESEARCH. Elsevier Science Ltd, New York, NY, 87:395-402, (2015).


The objective of this study was to investigate the anaerobic biodegradation of soybean biodiesel blends under methanogenic conditions. Biological methane potential (BMP) tests were conducted in serum bottles to determine the anaerobic biodegradation kinetics of biodiesel in the absence and presence of different concentrations of petrodiesel. The effect of petrodiesel on the biotransformation of biodiesel fractions in biodiesel/petrodiesel blends was determined by partitioning test. Furthermore, the possibility of anaerobic biodegradation of petrodiesel and its inhibition effects on the biodegradation of biodiesel was also investigated.


Biotransformation of soybean biodiesel and the inhibitory effect of petrodiesel were studied under methanogenic conditions. Biodiesel removal efficiency of more than 95% was achieved in a chemostat with influent biodiesel concentrations up to 2.45 g/L. The kinetics of anaerobic biodegradation of soybean biodiesel B100 (biodiesel only) with different petrodiesel loads were studied using biomass pre-acclimated to B100 and B80 (80% biodiesel and 20 petrodiesel). The results indicated that the biodiesel fraction of the blend could be effectively biodegraded, whereas petrodiesel was not biodegraded at all under methanogenic conditions. The presence of petrodiesel in blends with biodiesel had a greater inhibitory effect on the rate of biodegradation than the biodegradation efficiency (defined as the efficiency of methane production). Both the biodegradation rate coefficient and the methane production efficiency increased almost linearly with the increasing fraction of biodiesel. With the increasing fraction of petrodiesel, the biodegradation rate and efficiency were correlated with the concentration of soluble FAMEs in the water.

Record Details:

Product Published Date: 12/15/2015
Record Last Revised: 05/05/2017
OMB Category: Other
Record ID: 336169