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Applicability of integrated cell culture quantitative PCR (ICC-qPCR) for the detection of infectious adenovirus type 2 in UV disinfection studies
Ryu, H., J. Cashdollar, Shay Fout, K. A. Schrantz, AND Sam Hayes. Applicability of integrated cell culture quantitative PCR (ICC-qPCR) for the detection of infectious adenovirus type 2 in UV disinfection studies. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH, PART A. Taylor & Francis Group, London, Uk, 50(8):777-787, (2015).
This article describes the application of a novel adenovirus infectivity assay to understanding how effective disinfectants are for inactivating these viruses. This study investigated the effectiveness of ultra violet light energy to inactivate adenovirus 2 viruses.
Human adenovirus is relatively resistant to UV radiation and has been used as a conservative testing microbe for evaluations of UV disinfection systems as components of water treatment processes. In this study, we attempted to validate the applicability of integrated cell culture quantitative PCR (ICC-qPCR) as an alternative to the traditional cell culture method specifically with human adenovirus serotype 2 (AdV2) in a low-pressure UV disinfection study and to further optimize the procedures of ICC-qPCR for 24-well plate format. A two-day post-inoculation period and a maximum spiking level of 105 MPN/mL were selected as optimum conditions of ICC-qPCR with the tested AdV2. An approximate 1:1 correlation of virus quantities by the traditional and ICC-qPCR cell culture based methods suggested that ICC-qPCR is a satisfactory alternative for practical application in AdV2 disinfection studies. ICC-qPCR results, coupled with a first-order kinetic model (i.e., the inactivation rate constant of 0.0238 cm2mJ-1), showed that an UV dose of 168 mJ/cm2 achieved a 4-log inactivation credit for AdV2. This estimate is comparable to other studies with AdV2 and other adenovirus respiratory serotypes. The newly developed and optimized ICC-qPCR shows much promise for further study on its applicability of other slow replicating viruses in disinfection studies.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL RISK MANAGEMENT RESEARCH LABORATORY
WATER SUPPLY AND WATER RESOURCES DIVISION
MICROBIAL CONTAMINANTS CONTROL BRANCH