Science Inventory

Human fecal source identification with real-time quantitative PCR

Citation:

SHANKS, O. C., L. PEED, M. SIVAGANESAN, R. A. HAUGLAND, AND E. C. CHERN. Human fecal source identification with real-time quantitative PCR. 2nd, Chapter 7, Paulsen I., Holmes A. (ed.), Environmental Microbiology, Methods in Molecular Biology Series. Humana Press Incorporated, Totowa, NJ, 1096:85-99, (2014).

Impact/Purpose:

To inform the public of a real-time quantitative PCR (qPCR) method that targets a Bacteroides dori human-associated genetic marker for the identification of human fecal pollution in ambient water samples.

Description:

Waterborne diseases represent a significant public health risk worldwide, and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a Bacteroides dori human-associated genetic marker for the identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control (IAC), and data analysis. https://doi.org/10.1007/978-1-62703-712-9_7

Record Details:

Record Type: DOCUMENT (BOOK CHAPTER)
Product Published Date: 01/15/2014
Record Last Revised: 03/29/2018
OMB Category: Other
Record ID: 235487

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

WATER SUPPLY AND WATER RESOURCES DIVISION

MICROBIAL CONTAMINANTS CONTROL BRANCH