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STANDARDIZATION AND VALIDATION OF METHODS FOR ENUMERATION OF FECAL COLIFORM AND SALMONELLA IN BIOSOLIDS
MECKES, M. C. STANDARDIZATION AND VALIDATION OF METHODS FOR ENUMERATION OF FECAL COLIFORM AND SALMONELLA IN BIOSOLIDS. Presented at Pathogen Equivalency Committee Retreat, College Corner, OH, September 20, 2005.
To inform the public
Current federal regulations require monitoring for fecal coliforms or Salmonella in biosolids destined for land application. Methods used for analysis of fecal coliforms and Salmonella were reviewed and a standard protocol was developed. The protocols were then evaluated by testing various types of biosolids at a dozen laboratories throughout the U.S. and comparing results. Two methods were evaluated for fecal coliforms in biosolids, the lauryl tryptose broth (LTB)-EC broth method and the A-1 method. Both of these methods are multiple fermentation tube methods. A single method for Salmonella was evaluated: the modified semi-solid Rapaport-Vasiliadis procedure. Results of testing showed that there was considerable variation between laboratories depending upon the class of biosolids being evaluated. This presentation discusses the approach taken for these tests and some of the concerns regarding enumeration of fecal coliforms using these protocols.