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DECONTAMINATION ASSESSMENT OF BACILLUS ANTHRACIS, BACILLUS SUBTILIS, AND GEOBACILLUS STEAROTHERMOPHILUS SPORES ON INDOOR SURFACTS USING A HYDROGEN PERIOXIDE GAS GENERATOR
ROGERS, J. V., C. SABOURIN, Y. CHOI, W. RICHTER, D. HOLMES, K. RIGGS, M. TAYLOR, AND J. C. CHANG. DECONTAMINATION ASSESSMENT OF BACILLUS ANTHRACIS, BACILLUS SUBTILIS, AND GEOBACILLUS STEAROTHERMOPHILUS SPORES ON INDOOR SURFACTS USING A HYDROGEN PERIOXIDE GAS GENERATOR. DOI10.1111/j.1365-26, A. Gilmour (ed.), JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, 99(4):739-748, (2005).
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Aims: To evaluate the decontamination of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus spores on indoor surface materials using hydrogen peroxide gas. Methods and Results: B. anthracis, B. subtilis, and G. Stearothermophilus spores were dried on seven types of indoor surfaces and exposed to 1000 ppm hydrogen perioxide gas for 20 minutes. Hydrogen peroxide exposure significantly decreased viable B. anthracis, B. ssubtilis, and G. stearothermophilus spores on all test materials with the exception of G. stearothermophilus on industrial carpet. Significant differences were observed when comparing the reduction in viable spores of B. anthracis with both surrogates. The effectiveness of gaseous hydrogen peroxide on the growth of biological indicators and spore strips was evaluated in parallel with the test materials as a qualitative assessment of decontamination. AT one and seven day post-exposure decontaminated biological indicators and spore strips exhibited no growth, while the non-decontaminated samples displayed growth. Conclusions: Significant differences in decontamination efficacy of hydrogen peroxide gas on porous and non-porous surfaces were observed when comparing the mean log reduction in B. anthracis spores with B. subtilis and G. stearothermophilus spores. Significance and impact of study: These results provide new comparative information for the decontamination of B. anthracis spores with surrogates on indoor surfaces using hydrogen peroxide gas.