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ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCAROMYCES CEREVISIAE
Chen, C., T. G. Turi, D. Sanglard, AND J. C. Loper. ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCAROMYCES CEREVISIAE. doi:10.1016/0006-291, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. Elsevier Science, New York, NY, 146(3):1311-1317, (1987).
We have isolated the gene for cytochrome P450 lanosterol 14-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3′ portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.