Science Inventory

IN VITRO CYTOTOXICITY OF BTEX METABOLITES IN HELA CELL LINES

Citation:

Shen, Y. AND C. West*. IN VITRO CYTOTOXICITY OF BTEX METABOLITES IN HELA CELL LINES. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, 34(3):229-234, (1998).

Impact/Purpose:

Information

Description:

Fuel leakage from underground storage tanks is a major source of groundwater contamination. Although the toxicity of regulated compounds such as benzene, toluene, ethylbenzene, and xylene (BTEX) are well recognized, the cytotoxicity of their metabolites has not been studied extensively. In this study, Hela cells, propagated at 37 degrees C in an atmosphere of 5% C02-95% air, served as a target for evaluation of cytotoxicity of BTEX metabolites 3-methylcatechol, 4-methylcatechol, 4-hydroxybenzoic acid, and 4-hydroxy-3-methoxybenzoic acid. The cells were exposed to different concentrations of the metabolites, which subsequently showed inhibition of cell growth and produced dose-related decreases in cell viability and cell protein content. The BTEX metabolites affected the levels of the polyarnines spermidien, spermine, and putrescine, which are known to be important in cell proliferation. The cytotoxic effects for these BTEX metabolites to Hela cells were 3-methylcatechol> 4-methylcatechol> 4-hydroxy-3-methoxybenzoic acid> 4-hydroxybenzoic acid.

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Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 12/31/1998
Record Last Revised: 09/16/2009
OMB Category: Other
Record ID: 111701

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

SUBSURFACE PROTECTION AND REMEDIATION DIVISION