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Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water
Raphael, B. H., M. Lautenschlager, A. Kahler, S. Pai, B. A. Parks, S. R. Kalb, S. E. Maslanka, S Shah, M. Magnuson, AND V. R. Hill. Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water. Sept 2012 JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 90(3):267-272, (2012).
The purpose of this study was to adapt and evaluate the Botulinum Toxin Enzyme Linked Immunosorbant Assay (ELISA) (13) and Endopep-MS (3, 16) for use with drinking water samples. A further purpose of this study was to investigate the ability to concentrate toxin from large volumes of water (100-L) using ultrafiltration (UF) was evaluated in order to analyze otherwise undetectable levels of toxin.
URLs/Downloads:ULTRAFILTRATION IMPROVES ELISA AND ENDOPEP MS ANALYSIS OF BOTULINUM NEUROTOXIN TYPE A IN DRINKING WATER Exit
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HOMELAND SECURITY RESEARCH CENTER
THREAT AND CONSEQUENCE ASSESSMENT DIVISION