Office of Research and Development Publications

Development of a Sensitive Detection Method for Stressed E. coli O157:H7 in Source and Finished Drinking Water by Culture-qPCR

Citation:

Sen, K., J. L. Sinclair, L. Boczek, AND E. W. RICE. Development of a Sensitive Detection Method for Stressed E. coli O157:H7 in Source and Finished Drinking Water by Culture-qPCR. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, 45(7):2250-2256, (2011).

Impact/Purpose:

A sensitive and specific method that also demonstrates viability is of interest for detection of E. coli O157:H7 in drinking water. A combination of culture and qPCR was investigated. Two triplex qPCRs, one from a commercial source and another designed for this study were optimized from 5 different assays to be run on a single qPCR plate.

Description:

Journal article

URLs/Downloads:

DEVELOPMENT OF A SENSITIVE DETECTION METHOD FOR STRESSED E. COLI O157:H7 IN SOURCE AND FINISHED DRINKING WATER BY CULTURE-QPCR   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 02/22/2011
Record Last Revised: 11/28/2012
OMB Category: Other
Record ID: 234106

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HOMELAND SECURITY RESEARCH CENTER

WATER INFRASTRUCTURE PROTECTION DIVISION