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Development of a Sensitive Detection Method for Stressed E. coli O157:H7 in Source and Finished Drinking Water by Culture-qPCR
Sen, K., J. L. Sinclair, L. Boczek, AND E. W. RICE. Development of a Sensitive Detection Method for Stressed E. coli O157:H7 in Source and Finished Drinking Water by Culture-qPCR. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, 45(7):2250-2256, (2011).
A sensitive and specific method that also demonstrates viability is of interest for detection of E. coli O157:H7 in drinking water. A combination of culture and qPCR was investigated. Two triplex qPCRs, one from a commercial source and another designed for this study were optimized from 5 different assays to be run on a single qPCR plate.
URLs/Downloads:DEVELOPMENT OF A SENSITIVE DETECTION METHOD FOR STRESSED E. COLI O157:H7 IN SOURCE AND FINISHED DRINKING WATER BY CULTURE-QPCR Exit
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HOMELAND SECURITY RESEARCH CENTER
WATER INFRASTRUCTURE PROTECTION DIVISION