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BIOAUGMENTATION WITH BURKHOLDERIA CEPACIA PR1301 FOR IN SITU BIOREMEDIATION OF TRICHLOROETHYLENE CONTAMINATED GROUNDWATER (RESEARCH BRIEF)
McCarty, P. L., G. D. Hopkins, J. MunakataMarr, V. G. Matheson, M. E. Dolan, L. B. Dion, M. S. Shields, L. J. Forney, AND J. M. Tiedje. BIOAUGMENTATION WITH BURKHOLDERIA CEPACIA PR1301 FOR IN SITU BIOREMEDIATION OF TRICHLOROETHYLENE CONTAMINATED GROUNDWATER (RESEARCH BRIEF). U.S. Environmental Protection Agency, National Health and Environmental Effects Research, Gulf Ecology Division, Gulf Breeze, FL, EPA/600/S-98/001, 1998.
The objectives of this study were (1) to evaluate at laboratory and field scale the potential for bioaugmentation with a bacterial mutant containing a constitutive monooxygenase to enhance and improve in situ bioremediation of groundwater contaminated with TeE, (2) to determine the movement, fate, and effectiveness of introduced microorganisms in an aquifer, (3) to 2 evaluate the value of introduced microorganisms and of bioaugmentation for enhancing in situ biodegradation, and (4) to evaluate the applicability of molecular tools in the monitoring, operation, and control of in situ bioreclamation systems.
A pilot field study was conducted at the Moffett Federal Airfield, Mountain View, California, to determine whether effective in-situ aerobic cometabolic biodegradation of TCE could be accomplished through bioaugmentation with a genetically modified strain of Burkholderia cepacia G4 (G4) together with feeding of lactate to serve as an energy and growth substrate for the organism. Strain G4 is highly effective at TCE cometabolism but requires either phenol or toluene to induce oxygenase enzyme activity. A strain of G4 was developed through NTG mutagenesis that constitutively expresses the oxygenase so that no inducer need be added. The modified strain B. cepacia PR1301 (PR1301), can degrade TCE effectively while growing on simple substrates such as lactate. Strain-specific molecular probes were developed for monitoring the presence and movement of PR1301, and were based upon rep-PCR analysis.
Record Details:Record Type: DOCUMENT (PUBLISHED REPORT/REPORT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY
GULF ECOLOGY DIVISION