Science Inventory

ASSESSMENT OF TOXICANT-INDUCED ALTERATIONS IN OVARIAN STEROIDOGENESIS: A METHODOLOGICAL OVERVIEW

Citation:

Goldman, J M., S C. Laws, AND R L. Cooper. ASSESSMENT OF TOXICANT-INDUCED ALTERATIONS IN OVARIAN STEROIDOGENESIS: A METHODOLOGICAL OVERVIEW. Chapter 11, P. Hoyer (ed.), Ovarian Toxicology. CRC Press LLC, Boca Raton, FL, (2004).

Description:

RTD-03-035

Assessment of Toxicant-induced Alterations in Ovarian Steroidogenesis:
A Methodological Overview

Jerome M. Goldman, Susan C. Laws and Ralph L. Cooper

Abstract

A variety of methodological approaches have been used for the assessment of toxicant-induced alterations in ovarian steroidogenesis. For the purposes of the present overview, they have been categorized by (1) a combination of the type of toxicant exposure and steroid sampling employed (i.e., in vivo / in vivo, in vitro / in vitro or in vivo / in vitro), and (2) the type of biological material used for evaluation (i.e., whole or minced ovaries, intact follicles, or isolated cell preparations). All have both strengths and limitations, and which is selected for use will depend on a variety of factors, including specificity, cost, animal usage, technical difficulty and the extent to which metabolism of the compound contributes to its impact on steroid production. A discussion of these factors is included, along with criteria that must be taken under consideration when ovarian steroidogenesis is a component of a screening approach for the detection of potential endocrine-disrupting chemicals.

Record Details:

Record Type: DOCUMENT (BOOK CHAPTER)
Product Published Date: 05/01/2004
Record Last Revised: 12/22/2005
Record ID: 83132

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

REPRODUCTIVE TOXICOLOGY DIVISION

ENDOCRINOLOGY BRANCH