Science Inventory

RATE AND CAPACITY OF HEPATIC MICROSOMAL RING HYDROXYLATION OF PHENOL TO HYDROQUINONE AND CATECHOL IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Citation:

Kolanczyk, R C. AND P. K. Schmieder. RATE AND CAPACITY OF HEPATIC MICROSOMAL RING HYDROXYLATION OF PHENOL TO HYDROQUINONE AND CATECHOL IN RAINBOW TROUT (ONCORHYNCHUS MYKISS). TOXICOLOGY 176:77-90, (2002).

Description:

Rainbow trout liver microsomes were used to study the rate of ring-hydroxylation of phenol (PH) by directly measuring the production of hydroquinone (HQ), the primary metabolite, and catechol (CAT), a secondary metabolite. An HPLC method with integrated ultroviolet (UV) and electrochemical detection (ECD) was used for metabolite identification and quantification at low concentrations. - - -

These experiments provide a means to directly quantify metabolic bioactivation potential in fish species. This work initiates a series of studies to compare activation rate and capacity across aquatic species, providing a basis for the prediction of metabolic pathways and rates.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 06/21/2002
Record Last Revised: 12/22/2005
Record ID: 80647

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LABORATORY

MID-CONTINENT ECOLOGY DIVISION

MOLECULAR & CELLULAR MECHANISMS RESEARCH